Strelecková E, Kodetová D, Poucková P, Zadinová M, Lukás E, Rokyta R, Jirsa M
Clinic of Neurology, Charles University, Prague, Czech Republic.
Sb Lek. 1995;96(1):7-13.
The acute toxicity of specially purified meso-tetra-(4-sulfonatophenyl)-porphine (TPPS4) was measured in mice DBA/2 and Wistar rats after i.v. administration of the dye dissolved in saline solution. LD50 of our preparation was 352.5 mg/kg for mice and 574.9 mg/kg for rats. Motor nerve conduction velocity was measured in rats and rabbits in ether anesthesia N. ischiadicus and n. tibialis were stimulated by surface electrodes and M response was registered. No signs of neurotoxicity were found, even after application of 150 mg/kg b.w. of our TPPS4. Histologic evaluation was performed by routine method and standard electron microscopic procedure. Only minimal and sporadic axonal changes were found. The clinical application of low toxic TPPS4 was reconsidered.
在将溶解于盐溶液中的染料经静脉注射给DBA/2小鼠和Wistar大鼠后，测定了特殊纯化的中-四-(4-磺基苯基)-卟啉（TPPS4）的急性毒性。我们制剂的半数致死量（LD50）对小鼠为352.5毫克/千克，对大鼠为574.9毫克/千克。在乙醚麻醉下，用表面电极刺激大鼠和兔子的坐骨神经和胫神经，测量运动神经传导速度并记录M反应。即使应用150毫克/千克体重的我们的TPPS4，也未发现神经毒性迹象。通过常规方法和标准电子显微镜程序进行组织学评估。仅发现最小且偶发的轴突变化。重新考虑了低毒性TPPS4的临床应用。
