[Seasonal variation in the ultrastructure and calcium uptake rate of cardiac sarcoplasmic reticulum in ground sqirrel].

The ultrastructure and calcium uptake rate of the cardiac sarcoplasmic reticulum (SR) was quantatively compared between the ground squirrels (Citellus dauricus) in hibernating and non-hibernating season. (1) In our ultrastructural investigation, OsFeCN post-fixation method was used to enhance the staining of SR, which made the SR structure more recognizable. (2) The results of morphometrical analysis demonstrated that the volume density of SR and lipid droplet in the cardio-myocyte was significantly higher (P < 0.01) in the hibernating animal than in the summer active one, and the subcompartment of SR with this difference was non-junctional SR. (3) The cardiac SR from hibernating ground squirrels had a higher (P < 0.01) calcium uptake rate as measured by the absorbence of arsenazo-III Ca2+ indicator, than that from autumn active ones. Altogether, our results suggested that the function of SR in the uptake of cytosolic calcium was enhanced in hibernating season, which may play an important role in the cold tolerance of myocardium of a hibernator.