Yadav M, Hopwood V L, Multani A S, Mansfield P F, Takahashi Y, McIntyre B W, Udagawa T, Pathak S
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Anticancer Res. 1996 Jul-Aug;16(4A):1787-95.
By using Giemsa-banding and fluorescence in situ hybridization techniques, we have been able to identify primary and secondary cytogenetic abnormalities in four gastric tumors at different stages of development. Structural and numerical abnormalities were present in all four gastric tumors in chromosomes 3, 7, 11, and X. Other abnormalities involving chromosomes 1, 5, 6, 8, 13, 15, 17, 18, 19 and 22 were observed, but only in three advanced gastric tumors, suggesting that these were secondary/tertiary genetic defects. Based on these results it was possible for us to decipher primary and secondary genetic abnormalities in these four gastric tumors.
通过使用吉姆萨显带和荧光原位杂交技术，我们得以在四个处于不同发育阶段的胃肿瘤中识别出原发性和继发性细胞遗传学异常。在所有四个胃肿瘤中，3号、7号、11号染色体和X染色体均存在结构和数量异常。还观察到涉及1号、5号、6号、8号、13号、15号、17号、18号、19号和22号染色体的其他异常，但仅在三个进展期胃肿瘤中出现，这表明这些是继发性/三级基因缺陷。基于这些结果，我们得以解读这四个胃肿瘤中的原发性和继发性基因异常。
