Patel P S, Raval G N, Patel M M, Balar D B, Patel D D
Biochemistry Section, Gujarat Cancer Society Asarwa, Ahmedabad, India.
Anticancer Res. 1996 Jul-Aug;16(4A):2089-94.
Numerous investigators have identified, isolated and characterized serum glycoproteins that are claimed to be specifically associated with malignancy. We have carried out serum glycoprotein electrophoresis on polyacrylamide disc gel in 53 breast cancer patients, at diagnosis as well as during and after therapy. Follow-up samples were divided into complete responders (CR) (n = 138) and nonresponders (NR) (n = 44). Glycoprotein electrophoresis showed multiple bands for each sample which were categorized into four groups: albumin, alpha, beta and gamma. The results revealed a decreasing number of CR and increasing number of NR with elevated (as compared to pretreatment levels) albumin fraction glycoproteins. Gamma region glycoproteins showed the reverse pattern to that of albumin region glycoproteins. The alpha and beta region glycoproteins revealed an increasing number of CR having higher values with increase in follow-up duration. In comparison with their pretreatment values CR showed significantly increased (Paired "t" test) values of albumin, alpha and beta region glycoproteins (p < 0.01, p < 0.001 and p < 0.001, respectively) and decreased gamma region glycoproteins (p < 0.001). The albumin, alpha, beta and gamma region glycoprotein levels were comparable between NR and untreated cancer patients. The variations in albumin, alpha, beta and gamma region glycoproteins correlate with treatment response, which might be useful in the treatment monitoring, and prediction of recurrence in breast cancer patients.
许多研究人员已经鉴定、分离并表征了据称与恶性肿瘤特异性相关的血清糖蛋白。我们对53例乳腺癌患者在诊断时以及治疗期间和治疗后进行了聚丙烯酰胺圆盘凝胶血清糖蛋白电泳。随访样本分为完全缓解者（CR）（n = 138）和无反应者（NR）（n = 44）。糖蛋白电泳显示每个样本有多个条带，这些条带分为四组：白蛋白、α、β和γ。结果显示，随着白蛋白部分糖蛋白升高（与治疗前水平相比），CR数量减少，NR数量增加。γ区糖蛋白呈现出与白蛋白区糖蛋白相反的模式。α和β区糖蛋白显示，随着随访时间的增加，CR数量增加且值更高。与治疗前值相比，CR的白蛋白、α和β区糖蛋白值显著增加（配对“t”检验）（分别为p < 0.01、p < 0.001和p < 0.001），γ区糖蛋白值降低（p < 0.001）。NR和未治疗的癌症患者之间的白蛋白、α、β和γ区糖蛋白水平相当。白蛋白、α、β和γ区糖蛋白的变化与治疗反应相关，这可能有助于乳腺癌患者的治疗监测和复发预测。
