Pardigol A, Mägert H J, Hill O, Forssmann W G
Lower Saxony Institute for Peptide Research, Hannover, Germany.
Biochem Biophys Res Commun. 1996 Jul 25;224(3):638-44. doi: 10.1006/bbrc.1996.1078.
Guanylin (GCAP-I, guanylate cyclase activating peptide I) and uroguanylin (GCAP-II, guanylate cyclase activating peptide II) are regulatory peptides involved in the regulation of the intestinal chloride / water balance. They share significant structural homology to the E. coli enterotoxin STa, which binds to the particulate guanylyl cyclase C causing diarrhea in mammals. In this study we report the functional analysis of the guanylin / GCAP-I gene promoter region. By means of the luciferase reporter gene assay, we demonstrate a strong promoter activity in T84 cells. Especially the first 160 bp of the 5'-flanking region of the gene seem to be essential for gene induction. Our findings are the basis for further identification of important regulatory elements of the corresponding gene.
鸟苷林（GCAP-I，鸟苷酸环化酶激活肽I）和尿鸟苷林（GCAP-II，鸟苷酸环化酶激活肽II）是参与调节肠道氯/水平衡的调节肽。它们与大肠杆菌肠毒素STa具有显著的结构同源性，STa与颗粒性鸟苷酸环化酶C结合可导致哺乳动物腹泻。在本研究中，我们报告了鸟苷林/GCAP-I基因启动子区域的功能分析。通过荧光素酶报告基因检测，我们证明了其在T84细胞中有很强的启动子活性。特别是该基因5'侧翼区域的前160 bp似乎对基因诱导至关重要。我们的发现为进一步鉴定相应基因的重要调控元件奠定了基础。
