Yarwood S J, Kilgour E, Anderson N G
Hannah Research Institute, Scotland, United Kingdom.
Biochem Biophys Res Commun. 1996 Jul 25;224(3):734-9. doi: 10.1006/bbrc.1996.1092.
The effects of cyclic AMP on mitogen-activated protein (MAP) kinase activation were investigated in 3T3-F442A preadipocytes. Several agents, which raise intracellular cyclic AMP levels by distinct mechanisms, induced a transient activation of the p42 and p44 isoforms of MAP kinase and of a MAP kinase kinase. Activation of MAP kinase by cyclic AMP was prevented by two distinct inhibitors of cyclic AMP-dependent protein kinase and by PD 098059, a specific inhibitor of the activation of the MAP kinase kinase MEK 1. Therefore, in contrast to most cell types studied, cyclic AMP exerts a positive influence on the MAP kinase pathway in 3T3-F442A preadipocytes at a level upstream of the activation of MAP kinase kinase.
在3T3-F442A前脂肪细胞中研究了环磷酸腺苷（cAMP）对丝裂原活化蛋白（MAP）激酶激活的影响。几种通过不同机制提高细胞内环磷酸腺苷水平的试剂，可诱导MAP激酶的p42和p44亚型以及一种MAP激酶激酶的瞬时激活。环磷酸腺苷依赖性蛋白激酶的两种不同抑制剂以及MAP激酶激酶MEK 1激活的特异性抑制剂PD 098059可阻止cAMP对MAP激酶的激活。因此，与大多数研究的细胞类型不同，在3T3-F442A前脂肪细胞中，环磷酸腺苷在MAP激酶激酶激活的上游水平对MAP激酶途径产生积极影响。
