Velasco G, Sánchez C, Geelen M J, Guzmán M
Department of Biochemistry and Molecular Biology I, Faculty of Biology, Complutense University, Madrid, Spain.
Biochem Biophys Res Commun. 1996 Jul 25;224(3):754-9. doi: 10.1006/bbrc.1996.1095.
The present work was undertaken to test whether cytoskeletal components are involved in the control of rat-liver carnitine palmitoyltransferase I (CPT-I) activity by cellular effectors. The microtubule stabilizer taxol abolished the changes in CPT-I activity induced by the effectors tested. Taxol also prevented OA-induced shrinkage of hepatocytes as well as the enhanced release of lactate dehydrogenase from digitonin-permeabilized hepatocytes. On the basis of its relative sensitivity to tautomycin and OA, the modulation of CPT-I activity seemed to involve mostly protein phosphatase 1. These data suggest that the short-term control of hepatic CPT-I by cellular effectors may involve modulation of interactions between CPT-I and cytoskeletal components.
开展本研究以测试细胞骨架成分是否参与细胞效应物对大鼠肝脏肉碱棕榈酰转移酶I（CPT-I）活性的调控。微管稳定剂紫杉醇消除了所测试效应物诱导的CPT-I活性变化。紫杉醇还可防止油酸诱导的肝细胞萎缩以及从洋地黄皂苷通透化的肝细胞中增强释放乳酸脱氢酶。基于其对 tautomycin 和油酸的相对敏感性，CPT-I 活性的调节似乎主要涉及蛋白磷酸酶1。这些数据表明，细胞效应物对肝脏CPT-I的短期调控可能涉及CPT-I与细胞骨架成分之间相互作用的调节。
