Atanasova E, Todorov S, Noeva A
Institute of Physiology, Bulgarian Academy of Sciences.
Eksp Med Morfol. 1994;32(1-2):13-23.
Experiments were carried out on longitudinal and circular smooth-muscle strips isolated from the ileum and colon of healthy dogs (controls) and of dogs with local peritonitis. The changes in the contractile responses of the strips to carbachol administered cumulatively were studied, and the EC50 and pD2 values were calculated from the dose-response curves. For the colonic longitudinal strips the EC50 was 6 x 10(-8)M and the pD2 was 7.58. The pD2 for the ileal longitudinal strips was 7.83. The pD2 value for the circular strips from the ileum and colon was 7.19 and 8.87 respectively. In the dogs with local peritonitis, the sensitivity of the longitudinal strips to carbachol was higher (the pD2 for the ileum was 7.86 and for the colon-7.21) as compared to the circular strips (the pD2 for the ileum was 6.22 and for the colon-6.65). The affinity of the cholinoreceptors in the ileal circular strips to carbachol was decreased as compared to controls.
对从健康犬（对照组）以及患有局部腹膜炎的犬的回肠和结肠分离出的纵行和平滑肌环行肌条进行了实验。研究了肌条对累积给予的卡巴胆碱的收缩反应变化，并根据剂量-反应曲线计算了半数有效浓度（EC50）和亲和力指数（pD2）值。对于结肠纵行肌条，EC50为6×10⁻⁸M，pD2为7.58。回肠纵行肌条的pD2为7.83。回肠和结肠环行肌条的pD2值分别为7.19和8.87。在患有局部腹膜炎的犬中，与环行肌条相比，纵行肌条对卡巴胆碱的敏感性更高（回肠的pD2为7.86，结肠的为7.21）（回肠的pD2为6.22，结肠的为6.65）。与对照组相比，回肠环行肌条中胆碱能受体对卡巴胆碱的亲和力降低。
