Baranova N A, Krĕier V G, Egorov N S
Moscow State University.
Antibiot Khimioter. 1995 Aug;40(8):12-6.
The action of lovastatin, a competing inhibitor of 3-hydroxy-3-methylglutaryl CoA reductase, on bacterial bioluminescence was studied. The lovastatin lactone form and sodium salt of mevinolinic acid inhibited bacterial luciferase in vitro but did not affect bioluminescence of the intact cells of the luminous bacteria. The inhibition was found to be of a competing character in regard to aliphatic aldehyde, the bacterial luciferase substrate. Conditions under which the bioluminescence inhibition was proportional to the lovastatin concentration in the incubation mixture and a bioluminescence method for quantitative determination of the inhibitor were developed.
研究了3-羟基-3-甲基戊二酰辅酶A还原酶的竞争性抑制剂洛伐他汀对细菌生物发光的作用。洛伐他汀内酯形式和莫纳可林酸的钠盐在体外抑制细菌荧光素酶，但不影响发光细菌完整细胞的生物发光。发现该抑制作用在脂肪醛（细菌荧光素酶底物）方面具有竞争性。确定了生物发光抑制与孵育混合物中洛伐他汀浓度成比例的条件，并开发了一种用于定量测定该抑制剂的生物发光方法。
