Jen C J, Dong H P, Chen H, Wing L C, Tang M J, Chang W C, Lin M T, Shi G Y
Department of Physiology, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China.
Thromb Haemost. 1996 Jan;75(1):101-6.
Thrombolytic therapy is known to induce platelet-related side effects. We used a parallel-plate flow chamber, which was connected to the femoral artery of the rat, to measure platelet adhesion ex vivo. A collagen-coated arterioarterial shunt between two carotid arteries was used to measure shunt patency duration as an index of antithrombotic efficacy. Tissue-type plasminogen activator (t-PA), vitamin E, and the combination of these two were intravenously administered for 60 min. Measurements were performed before drug administration, and at 30, 60, 120 min after the initiation of drug infusion. Our results indicated that (1) treatments with t-PA or t-PA/vitamin E prolonged the time to shunt occlusion at 30 and 60 min; (2) t-PA enhanced platelet adhesion at 60 and 120 min; (3) vitamin E tended to reduce platelet adhesion; (4) t-PA/vitamin E reduced the t-PA-enhanced platelet adhesion; (5) at the high-density area of platelet adhesion under t-PA treatment, the adherent platelets demonstrated severe morphological changes which could be blocked by vitamin E. These data suggest that t-PA may enhance platelet adhesion in rats and that this adverse effect can be suppressed by co-administration of vitamin E.
已知溶栓疗法会引发与血小板相关的副作用。我们使用一个连接到大鼠股动脉的平行板流动腔来体外测量血小板黏附。使用两根颈动脉之间的胶原包被的动脉 - 动脉分流管来测量分流管通畅持续时间，以此作为抗血栓疗效的指标。组织型纤溶酶原激活剂（t-PA）、维生素E以及这两者的组合进行静脉注射60分钟。在给药前以及药物输注开始后的30、60、120分钟进行测量。我们的结果表明：（1）t-PA或t-PA/维生素E治疗在30和60分钟时延长了分流管闭塞时间；（2）t-PA在60和120分钟时增强了血小板黏附；（3）维生素E倾向于减少血小板黏附；（4）t-PA/维生素E降低了t-PA增强的血小板黏附；（5）在t-PA治疗下血小板黏附的高密度区域，黏附的血小板表现出严重的形态变化，而这种变化可被维生素E阻断。这些数据表明t-PA可能增强大鼠的血小板黏附，并且这种不良反应可通过联合使用维生素E来抑制。
