Kumar Soni S, Venkateswara Rao M, Das D
Chemical Engineering Department, Indian Institute of Technology, Kharagpur.
Indian J Exp Biol. 1995 Dec;33(12):957-61.
Efficiency of an enzymatic starch saccharification process depends not only on the activity of the glucoamylase but also its purity. About 96.82% unwanted proteins present in 2-day old culture broth of A. awamori NRRL-3112 (grown in MYGP medium) were separated by precipitation with ammonium sulphate which was followed by dialysis. More than 80% activity of the glucoamylase was recovered. Three protein fractions (A, B, C) were identified using gel permeation chromatography. Fractions A and B showed comparatively higher glucoamylase activity than fraction-C. Moreover, fraction-B showed no product inhibition. The optimum temperature and pH of the purified enzyme (fraction-B) were 60 degrees C and 4.0 respectively. The saccharification efficiency of potato pulp was more in case of using purified glucoamylase (fraction-B) as compared to that of crude enzyme.
酶促淀粉糖化过程的效率不仅取决于葡萄糖淀粉酶的活性，还取决于其纯度。通过硫酸铵沉淀随后透析，分离出了泡盛曲霉NRRL - 3112（在MYGP培养基中培养）2日龄培养液中约96.82%的不需要的蛋白质。葡萄糖淀粉酶的活性回收率超过80%。使用凝胶渗透色谱法鉴定出三个蛋白质组分（A、B、C）。组分A和B的葡萄糖淀粉酶活性比组分C相对更高。此外，组分B没有产物抑制作用。纯化酶（组分B）的最适温度和pH分别为60℃和4.0。与粗酶相比，使用纯化的葡萄糖淀粉酶（组分B）时，马铃薯浆的糖化效率更高。
