Fingerová H, Petrová P, Strnad M
Department of Obstetrics and Gynaecology, Palacký University, Olomouc, Czech Republic.
Acta Univ Palacki Olomuc Fac Med. 1994;138:21-3.
A sensitive radioimmunoassay (RIA) for the determination of erythropoietin (Epo) in blood serum and amniotic fluid has been developed and validated. The assay uses a polyclonal antibody obtained in rabbits immunized with recombinant human Epo by one of the authors. Tracer and second antibody are commercially available. With 100 microliters of sample, the assay can detect 3 U/l. The reproducibility of the assay compares well with commercial Epo RIAs. The normal values estimated in a group of blood donors are slightly higher than those reported for various RIA determinations. The clinical usefulness of the assay was demonstrated by observing the expected increases or decreases of Epo levels in various clinical states known to alter Epo production. The assay meets all requirements of a routine clinical assay at significantly reduced costs, which makes it particularly suitable for use in research applications.
已开发并验证了一种用于测定血清和羊水中促红细胞生成素（Epo）的灵敏放射免疫测定法（RIA）。该测定法使用了由作者之一用重组人促红细胞生成素免疫兔子获得的多克隆抗体。示踪剂和二抗可从市场购得。使用100微升样品，该测定法可检测到3 U/l。该测定法的重现性与市售的促红细胞生成素放射免疫测定法相当。一组献血者中估计的正常值略高于各种放射免疫测定法报告的正常值。通过观察已知会改变促红细胞生成素产生的各种临床状态下促红细胞生成素水平的预期升高或降低，证明了该测定法的临床实用性。该测定法以显著降低的成本满足了常规临床测定的所有要求，这使其特别适合用于研究应用。
