基于聚合酶链反应的磁珠定点诱变
Polymerase chain reaction-based site-directed mutagenesis using magnetic beads.
作者信息
Fieschi J, Niccoli P, Camilla C, Chames P, Chartier M, Baty D
机构信息
Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, Institut de Biologie Structurale et de Microbiologie, CNRS, Marseille, France.
出版信息
Anal Biochem. 1996 Feb 15;234(2):210-4. doi: 10.1006/abio.1996.0074.
Thyroglobulin double mutants with various substitutions were obtained with a new polymerase chain reaction-based mutagenesis technique. Maximum length of megaprimer and efficiency of mutagenesis were improved by purification of single-stranded DNA, using the avidin-biotin interaction. This method might allow the construction of large libraries of DNA, mutated at different sites.
利用一种基于聚合酶链反应的新诱变技术获得了具有各种取代的甲状腺球蛋白双突变体。通过利用抗生物素蛋白-生物素相互作用纯化单链DNA，提高了大引物的最大长度和诱变效率。该方法可能允许构建在不同位点发生突变的大型DNA文库。
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