Boyd R B, Thompson V W, Atkin J
Department of Biomedical Sciences, Pennsylvania College of Podiatric Medicine, Philadelphia, USA.
J Am Podiatr Med Assoc. 1996 Feb;86(2):57-62. doi: 10.7547/87507315-86-2-57.
The alteration in glomerular basement membrane permeability associated with microangiopathy in streptozotocin-induced diabetic rats was studied by determining the movement across the glomerular basement membrane of anionic ferritin probes injected into rats at different points in the development of the disease. Visualization of the concentration gradient of anionic ferritin and changes in ultrastructure was accomplished by electron microscopic examination of renal tissue prepared from both control and diabetic rats. In all control rats, the anionic ferritin did not leave the glomerular capillary lumen, nor were there any changes in the normal morphology of the glomerular capillary wall. In the diabetic animals, the concentration of anionic ferritin shifted from the capillary lumen in the abluminal direction. Distinct morphologic changes, such as widening of endothelial intercellular junctions, focal detachment of podocyte foot processes, and extensive thickening of the glomerular basement membrane, were noted in the diabetic rat, and these changes appear to correlate with the observed increase in permselectivity of anionic ferritin across the glomerular capillary wall.
通过测定在链脲佐菌素诱导的糖尿病大鼠疾病发展的不同阶段注入大鼠体内的阴离子铁蛋白探针穿过肾小球基底膜的移动情况，研究了与微血管病变相关的肾小球基底膜通透性改变。通过对对照大鼠和糖尿病大鼠制备的肾组织进行电子显微镜检查，实现了阴离子铁蛋白浓度梯度的可视化和超微结构的变化。在所有对照大鼠中，阴离子铁蛋白未离开肾小球毛细血管腔，肾小球毛细血管壁的正常形态也未发生任何变化。在糖尿病动物中，阴离子铁蛋白的浓度从毛细血管腔向管腔外方向转移。在糖尿病大鼠中观察到明显的形态学变化，如内皮细胞间连接增宽、足细胞足突局灶性脱离和肾小球基底膜广泛增厚，这些变化似乎与观察到的阴离子铁蛋白跨肾小球毛细血管壁的选择通透性增加相关。
