Gillen C, Gleichmann M, Greiner-Petter R, Zoidl G, Kupfer S, Bosse F, Auer J, Müller H W
Department of Neurology, University of Düsseldorf, Moorenstrasse 5,D-40558 Düsseldorf, Germany.
Eur J Neurosci. 1996 Feb;8(2):405-14. doi: 10.1111/j.1460-9568.1996.tb01223.x.
We have isolated a 1.476 bp cDNA (NTII11) representing a transcript that is differntially expressed during sciatic nerve development and regeneration in the rat. Nucleotide sequence comparison indicates partial identity with a recently isolated plasmolipin cDNA. However, our clone extends the published sequence by 234 bp at the 5' end and predicts a protein that contains an additional 25 amino acids at th N-terminus. The open reading frame of th NTII11 transcript encodes a 19.4 kDa protein with four putative transmembrane domains. Northern blot analyses revealed a tissue-specific expression was confirmed by in situ hybridization, and cellular localization of plasmolipin mRNA was demonstrated in Schwann cells of the sciatic nerve and in glial cells of myelinated brain structures. The steady-state levels of plasmolipin mRNA were markedly altered (i) during development of sciatic nerve and brain. (ii) after sciatic nerve injury, and (ii) in cured Schwann cells maintained under different conditions of cell growth and arrest. Our data indicate a function of plasmolipin during myelination in the central as well as in the peripheral nervous system.
我们分离出了一个1476bp的cDNA（NTII11），它代表了一种在大鼠坐骨神经发育和再生过程中差异表达的转录本。核苷酸序列比较表明，它与最近分离出的质膜脂蛋白cDNA有部分同源性。然而，我们的克隆在5'端将已发表的序列延长了234bp，并预测了一个在N端含有另外25个氨基酸的蛋白质。NTII11转录本的开放阅读框编码一个19.4kDa的蛋白质，含有四个推定的跨膜结构域。Northern印迹分析显示了组织特异性表达，原位杂交证实了这一点，并且在坐骨神经的雪旺细胞和有髓脑结构的神经胶质细胞中证明了质膜脂蛋白mRNA的细胞定位。质膜脂蛋白mRNA的稳态水平在（i）坐骨神经和脑发育期间、（ii）坐骨神经损伤后以及（iii）在不同细胞生长和停滞条件下培养的雪旺细胞中发生了显著变化。我们的数据表明质膜脂蛋白在中枢和外周神经系统的髓鞘形成过程中发挥作用。
