Quist A P, Bergman A A, Reimann C T, Oscarsson S O, Sundqvist B U
Dept. of Radiation Sciences, Uppsala University, Sweden.
Scanning Microsc. 1995 Jun;9(2):395-400.
The most sensitive analytical techniques available today for detecting immuno assay complexes are radio or enzyme immuno analytical techniques, by which quantities of 10(7)-10(8) analyte molecules can be detected. With the introduction of scanning force microscopy, a new method for detecting biological processes became available. Here, we examine the feasibility of using scanning force microscopy as a biosensitive tool. We demonstrate that single or multiple rabbit anti-human serum albumin molecules form complexes with preadsorbed single human serum albumin molecules on mica. However, no interaction is observed between human immunoglobulin G molecules and preadsorbed single albumin molecules; only separate antigens and antibodies are observed at random positions on the mica. This shows the ability of scanning force microscopy to act as a biosensor for detection of immunocomplexes, and to act as a very powerful tool to study molecule-surface interactions in general.
当今用于检测免疫分析复合物的最灵敏分析技术是放射免疫分析技术或酶免疫分析技术，通过这些技术可以检测到数量为10⁷ - 10⁸的分析物分子。随着扫描力显微镜的引入，一种检测生物过程的新方法出现了。在此，我们研究了使用扫描力显微镜作为生物敏感工具的可行性。我们证明，单个或多个兔抗人血清白蛋白分子与预先吸附在云母上的单个人类血清白蛋白分子形成复合物。然而，未观察到人类免疫球蛋白G分子与预先吸附的单个白蛋白分子之间存在相互作用；在云母上的随机位置仅观察到分离的抗原和抗体。这表明扫描力显微镜有能力作为检测免疫复合物的生物传感器，并且总体上是研究分子 - 表面相互作用的非常强大的工具。
