De Wolf M, Hilderson H J, Lagrou A, Dierick W
Arch Int Physiol Biochim. 1978 Dec;86(5):1077-89. doi: 10.3109/13813457809055963.
Phospholipase and lysophospholipase activities are present in bovine thyroid (De Wolf et al., 1976). However, using exogenous [14C] phosphatidylcholine as substrate and subcellular fractions as enzyme source no activity could be detected at neutral and alkaline pH. Phospholipase A2 activity was found at neutral pH when [14C] phosphatidylethanolamine was substituted for [14C] phosphatidylcholine (De Wolf et al., 1976). In the present paper the occurrence of neutral and alkaline phospholipase A activities is clearly established. In addition their subcellular localization was investigated.
磷脂酶和溶血磷脂酶活性存在于牛甲状腺中（德·沃尔夫等人，1976年）。然而，以外源[14C]磷脂酰胆碱为底物，以亚细胞级分为酶源时，在中性和碱性pH条件下未检测到活性。当用[14C]磷脂酰乙醇胺替代[14C]磷脂酰胆碱时，在中性pH条件下发现了磷脂酶A2活性（德·沃尔夫等人，1976年）。在本文中，中性和碱性磷脂酶A活性的存在得到了明确证实。此外，还研究了它们在亚细胞中的定位。
