Konishi T, Nishio T, Kiyosawa S, Fujiwara Y, Konoshima T
Kyoto Pharmaceutical University, Japan.
Yakugaku Zasshi. 1996 Feb;116(2):148-57. doi: 10.1248/yakushi1947.116.2_148.
Four flavonoid compounds, avicularin (1), hyperin (2), quercitrin (3), taxillusin (4), were isolated from leaves of Taxillus kaempferi, and three catechin compounds, (+)-catechin (6), procyanidin B-1 (7), procyanidin B-3 (8), showing a reddish purple color on the reaction with Ehrlich reagent, were also isolated from the stems. Each of these seven isolated compounds were identified by comparing the spectral data with those of the references. Compound 8 was examined to substantiate the stereochemical configurations of conformer, a and b in the detailed 1H-nuclear magnetic resonance (1H-NMR) spectrum using the nuclear Overhauser effect (NOE) difference spectroscopy and spin decoupling experiment in the acetone-d6 solution.
从红杉叶中分离出四种黄酮类化合物，扁蓄苷（1）、金丝桃苷（2）、槲皮苷（3）、松萝苷（4），并且从茎中还分离出三种儿茶素化合物，（+）-儿茶素（6）、原花青素B-1（7）、原花青素B-3（8），它们与埃利希试剂反应时呈现红紫色。通过将这七种分离化合物的光谱数据与参考文献的数据进行比较，对每种化合物进行了鉴定。使用核Overhauser效应（NOE）差光谱法和丙酮-d6溶液中的自旋去耦实验，对化合物8进行了检测，以证实其构象异构体a和b在详细的1H-核磁共振（1H-NMR）光谱中的立体化学构型。
