螺旋-环-螺旋转录因子USF与人类淀粉样前体蛋白的基础启动子相互作用。

The helix-loop-helix transcription factor USF interacts with the basal promoter of human amyloid precursor protein.

作者信息

Bourbonnière M, Nalbantoglu J

机构信息

Department of Neurology and Neurosurgery, McGill University, Montreal, Que, Canada.

出版信息

Brain Res Mol Brain Res. 1996 Jan;35(1-2):304-8. doi: 10.1016/0169-328x(95)00208-a.

DOI:10.1016/0169-328x(95)00208-a

PMID:8717367

Abstract

Nuclear factors from HeLa, PC12, NG108-15 and SK-N-SH cell lines recognized an oligonucleotide (-56 to -37: APP-E1) containing an E box (CANNTG) which had previously been characterized as a DNase I-protected sequence in the basal promoter of the human amyloid precursor protein (APP) gene. Binding to APP-E1 was competed with an oligonucleotide encompassing the recognition site of the transcription factor USF. Antibodies directed against USF interacted with the APP-E1-protein complex and in vitro synthesized USF could bind APP-E1. Co-expression of USF cDNA transactivated a human APP-reporter gene construct. These results suggest that USF may play a role in the expression of the APP gene.

摘要

来自HeLa、PC12、NG108-15和SK-N-SH细胞系的核因子识别一种寡核苷酸（-56至-37：APP-E1），该寡核苷酸含有一个E盒（CANNTG），此E盒先前已被鉴定为人类淀粉样前体蛋白（APP）基因基础启动子中的一个DNase I保护序列。与APP-E1的结合可被包含转录因子USF识别位点的寡核苷酸竞争。针对USF的抗体与APP-E1-蛋白复合物相互作用，体外合成的USF能够结合APP-E1。USF cDNA的共表达可反式激活人APP报告基因构建体。这些结果表明，USF可能在APP基因的表达中发挥作用。