Kakkar P, Mehrotra S, Viswanathan P N
Ecotoxicology Section, Industrial Toxicology Research Centre, Lucknow, India.
Mol Cell Biochem. 1996 Jan 12;154(1):39-45. doi: 10.1007/BF00248459.
Tert-butyl hydroperoxide induced swelling of freshly isolated rat liver mitochondria was inhibited by butylated hydroxytoluene, butylated hydroxyanisole and alpha-tocopherol by acting at the initial phase. EDTA was more effective than EGTA in reducing the initial swelling and so were desferal and bipyrridyl. Spermine, an allosteric activator of calcium uptake, enhanced swelling whereas lanthanum and ruthenium red, the Ca2+ uniport blockers, reduced it. Inhibition of phospholipase A2 by dibucaine and Ca2+ activated proteases by antipain and leupeptin also reduced t-BHP induced swelling. The data indicate that peroxidative mitochondrial swelling involves an iron mediated initial rapid phase and a subsequent calcium dependent propagation phase.
叔丁基过氧化氢诱导的新鲜分离大鼠肝线粒体肿胀在初始阶段可被丁基化羟基甲苯、丁基化羟基茴香醚和α-生育酚抑制。乙二胺四乙酸（EDTA）在减轻初始肿胀方面比乙二醇双四乙酸（EGTA）更有效，去铁胺和联吡啶也是如此。精胺是钙摄取的变构激活剂，可增强肿胀，而镧和钌红是Ca2+单向转运体阻滞剂，可减轻肿胀。丁卡因对磷脂酶A2的抑制以及抗痛素和亮抑蛋白酶肽对Ca2+激活蛋白酶的抑制也可减轻叔丁基过氧化氢诱导的肿胀。数据表明，过氧化线粒体肿胀涉及铁介导的初始快速阶段和随后的钙依赖性传播阶段。
