HLA-B27 determination by polymerase chain reaction.
作者信息
Kilpatrick D C
出版信息
Dis Markers. 1996 Mar;12(4):247-51. doi: 10.1155/1996/184375.
PMID:8718784
Abstract
A method for determining the presence or absence of HLA-B27 by selective amplification of a region in the third exon of the HLA-B27 gene common to B2701 to B2705 inclusive, was evaluated. This polymerase chain reaction/sequence specific primer (PCR-SSP) method gave perfect correlation with serological typing on 40 individuals of previously determined HLA type and on 50 further clinical samples evaluated blind. It was concluded that HLA-B27 determination by PCR-SSP is simple, reliable, cost-effective and convenient for laboratory staff.
摘要
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