猫1型疱疹病毒的ICP4通过猫免疫缺陷病毒长末端重复序列（LTR）中的C/EBP位点下调LTR指导的基因表达。

The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat (LTR)-directed gene expression via the C/EBP site in the LTR.

作者信息

Kawaguchi Y, Maeda K, Pecoraro M R, Inoshima Y, Jang H K, Kohmoto M, Iwatsuki K, Ikeda Y, Shimojima M, Tohya Y

机构信息

Department of Veterinary Microbiology, University of Tokyo, Japan.

出版信息

J Vet Med Sci. 1995 Dec;57(6):1129-31. doi: 10.1292/jvms.57.1129.

DOI:10.1292/jvms.57.1129

PMID:8720064

Abstract

We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

摘要

我们通过在克兰德尔猫肾细胞中进行瞬时转染试验，研究了1型猫疱疹病毒（FHV-1）的ICP4对猫免疫缺陷病毒（FIV）长末端重复序列（LTR）指导的基因表达的影响。我们证明，在LTR中引入C/EBP位点的位点特异性突变后，FHV-1 ICP4能显著刺激FIV LTR，并且C/EBP位点足以赋予FHV-1 ICP4对异源启动子的抑制作用。这些结果表明，FHV-1 ICP4既具有反式激活FIV LTR指导的基因表达的能力，又具有通过C/EBP位点下调FIV LTR的能力。