Muramatsu T, Hatoko M, Tada H, Kobayashi N, Shirai T
Department of Dermatology, Nara Medical University, Japan.
J Dermatol. 1996 Jan;23(1):1-5. doi: 10.1111/j.1346-8138.1996.tb03960.x.
To examine the inducibility of 27-kD-molecular-weight heat shock protein (HSP27) in human skin, an indirect immunofluorescence (IF) study was performed on organ-cultured normal human skin by using a monoclonal antibody specific for HSP27. After heat treatment at 45 degrees C for 1 h, nuclear IF was observed in the epidermal cells. When the organ-cultured skin explants were exposed to 10.0 micrograms/ml or 100.0 micrograms/ml 8-methoxypsoralen (8-MOP) for 1 h and then irradiated with UVA (320-400 nm), positive nuclear IF was also observed 6 h after UVA irradiation. Considering these results with the previous reports about HSP72, it appears that, in human skin, HSP27 as well as HSP72 plays an important role in resisting various environmental stresses.
为检测27-kD分子量热休克蛋白（HSP27）在人皮肤中的诱导性，我们使用针对HSP27的单克隆抗体，对器官培养的正常人皮肤进行了间接免疫荧光（IF）研究。在45℃热处理1小时后，在表皮细胞中观察到核内免疫荧光。当将器官培养的皮肤外植体暴露于10.0微克/毫升或100.0微克/毫升的8-甲氧基补骨脂素（8-MOP）1小时，然后用紫外线A（320-400纳米）照射后，在紫外线A照射6小时后也观察到阳性核内免疫荧光。结合此前关于HSP72的报道来看这些结果，似乎在人皮肤中，HSP27以及HSP72在抵抗各种环境应激中发挥着重要作用。
