Fehr D, Bolla S, Herrewegh A A, Horzinek M C, Lutz H
Veterinär-Medizinisches Labor, Departement für Innere Veterinärmedizin, Universität Zürich und Institut für Veternär-Virologie.
Schweiz Arch Tierheilkd. 1996;138(2):74-9.
The aim of this study was to further investigate the pathogenesis and epidemiology of feline coronavirus (FCoV)-infections and among others to determine the prognostic value of a positive result in the RT-PCR for FCoV in serum samples collected from cats with abdominal signs. Viral RNA was isolated from 100 microl of serum and subsequently amplified by a nested RT-PCR using primers binding to a highly conserved region of the 3'-end of the FCoV-genome. Sixty-three serum samples collected from 62 cats with abdominal signs were examined by RT-PCR and the clinical outcome was followed up. Four of these cats with a positive PCR-result are healthy more than 70 months after the collection of the blood sample. It can be concluded that viremia with FCoV does not necessarily lead to FIP and death. With respect to diagnosing FIP, a positive FCoV-RT-PCR is of low prognostic and diagnostic value. It can not be recommended to use this assay as sole indication to euthanize cats. Further studies will have to be carried out to demonstrate if the prognostic and diagnostic value of this PCR-assay in other samples such as peripheral blood mononuclear cells is more reliable. However, this method was found to be an important tool to further study the pathogenesis and epidemiology of FIP.
本研究的目的是进一步调查猫冠状病毒（FCoV）感染的发病机制和流行病学，尤其要确定从有腹部症状的猫采集的血清样本中FCoV的逆转录聚合酶链反应（RT-PCR）阳性结果的预后价值。从100微升血清中分离病毒RNA，随后使用与FCoV基因组3'端高度保守区域结合的引物通过巢式RT-PCR进行扩增。对从62只患有腹部症状的猫采集的63份血清样本进行RT-PCR检测，并对临床结果进行随访。其中4只PCR结果呈阳性的猫在采集血样70多个月后仍然健康。可以得出结论，FCoV病毒血症不一定会导致猫传染性腹膜炎（FIP）和死亡。关于FIP的诊断，FCoV-RT-PCR阳性的预后和诊断价值较低。不建议将该检测作为安乐死猫的唯一指征。必须开展进一步研究，以证明该PCR检测在其他样本（如外周血单个核细胞）中的预后和诊断价值是否更可靠。然而，该方法被认为是进一步研究FIP发病机制和流行病学的重要工具。
