Dolf G, Colomb B, Schläpfer J, Leuenberger J, Märki U
Institut für Tierzucht, Universität Bern.
Schweiz Arch Tierheilkd. 1996;138(3):157-60.
Microsatellite analysis was applied to identify the inbred rat strains LEW, SHR and WKY. Of a set of seven commercially available primer pairs three pairs were used individually and two times two in duplex polymerase chain reactions (PCR). The products were analyzed in ethidium bromide stained agarose gels. The resulting banding patterns clearly demonstrated the homogeneity within strains and the differences between strains.
应用微卫星分析来鉴定近交系大鼠LEW、SHR和WKY。在一组七种市售引物对中，三对单独使用，两对用于双重聚合酶链反应（PCR）两次。产物在溴化乙锭染色的琼脂糖凝胶中进行分析。所得的条带模式清楚地表明了品系内的同质性以及品系间的差异。
