De Vita S, Ferraccioli G F, Dolcetti R, Marzotto A, Pivetta B, Pomponi F, Boiocchi M
Department of Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano (PN), Italy.
Clin Exp Rheumatol. 1996 Jan-Feb;14 Suppl 14:S21-9.
While the simple detection of B-cell clonality does not imply B-cell malignancy, comprehensive analyses of B-cell clonality are crucial to investigate the pathobiology of B-cell lymphoproliferative disorders. Our recent studies in patients with Sjögren's syndrome have highlighted how multiple molecular analyses of B-cell clonality (Southern blot, polymerase chain reaction, single strand conformation polymorphism, and DNA sequence analysis) in prelymphomatous lesions may be of great value in helping to define the stages of progression towards low-grade malignancy. The study of T-cell expansion may also be important in investigations of the pathobiology of the different stages of B-cell lymphoproliferation (fully benign, pseudolymphomatous, or definitely malignant), which may still be T-cell-and antigen/ autoantigen-dependent.
虽然单纯检测B细胞克隆性并不意味着B细胞恶性肿瘤，但对B细胞克隆性进行全面分析对于研究B细胞淋巴增殖性疾病的病理生物学至关重要。我们最近对干燥综合征患者的研究强调了对淋巴瘤前期病变中的B细胞克隆性进行多种分子分析（Southern印迹法、聚合酶链反应、单链构象多态性和DNA序列分析）在帮助确定向低度恶性进展阶段方面可能具有的巨大价值。对T细胞扩增的研究在B细胞淋巴增殖不同阶段（完全良性、假淋巴瘤性或明确恶性）的病理生物学研究中也可能很重要，这些阶段可能仍然依赖于T细胞以及抗原/自身抗原。
