恩韦肟与双羟萘酸对1型脊髓灰质炎病毒复制的协同抑制作用。

Synergistic inhibitory effect of enviroxime and disoxaril on poliovirus type 1 replication.

作者信息

Nikolaeva L, Galabov A S

机构信息

Department of Virology, Bulgarian Academy of Sciences, Sofia, Bulgaria.

出版信息

Acta Virol. 1995 Dec;39(5-6):235-41.

PMID:8722291

Abstract

The effects of enviroxime, disoxaril and ribavirin in pair combinations on poliovirus type 1 (Mahoney) replication in FL cells were tested. Beforehand, the fifty percent inhibitory concentration (IC50) was determined for each compound alone: enviroxime - 0.2 mumol/1, disoxaril - 0.3 mumol/1, ribavirin - 3 mumol/1. Combining enviroxime with disoxaril resulted in synergistic interaction, while combinations with ribavirin were markedly antagonistic. Enviroxime-and disoxaril-resistant poliovirus mutants appeared following 10 and 2 consecutive passages in FL cells, respectively. No cross-resistance was observed between these mutants towards disoxaril and enviroxime, respectively.

摘要

测试了环境肟、双异丙氧嘧啶和利巴韦林两两组合对1型脊髓灰质炎病毒（马奥尼株）在FL细胞中复制的影响。事先分别测定了每种化合物的半数抑制浓度（IC50）：环境肟为0.2μmol/1，双异丙氧嘧啶为0.3μmol/1，利巴韦林为3μmol/1。环境肟与双异丙氧嘧啶组合产生协同相互作用，而与利巴韦林组合则明显拮抗。分别在FL细胞中连续传代10次和2次后出现了对环境肟和双异丙氧嘧啶耐药的脊髓灰质炎病毒突变体。这些突变体之间分别对双异丙氧嘧啶和环境肟未观察到交叉耐药性。