Nutrient composition for cultivation of Japanese encephalitis virus in vitro.

Effects of certain groups of nutrients such as glucose, essential amino acids (AA), nonessential AA, vitamins and trace nutrients on the multiplication of various strains of Japanese encephalitis virus (JEV) were studied with an aim to optimise the conditions for cultivation of the virus in porcine stable (PS) kidney cell cultures. Eagle's Minimal Essential Medium (MEM) was modified by addition of the nutrients in different concentrations and combinations. Glucose was found the most important single nutrient in promoting significantly the virus multiplication. Essential AA alone did not influence the virus yield, while in combination with glucose they caused its marked increase. Vitamins and other nutrients did not stimulate significantly virus multiplication. The study revealed that the extent of the glucose effect depends on the virus strain used.