Ricker D D, Chamness S L, Hinton B T, Chang T S
Department of Population Dynamics, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland, USA.
J Androl. 1996 Mar-Apr;17(2):117-26.
Sympathetic denervation of the rat cauda epididymidis by surgical removal of the inferior mesenteric ganglion (IMG) results in an excessive accumulation of sperm in the cauda epididymidis as well as significant changes in cauda sperm motility and cauda epididymal gross histology. The objective of the present study was to determine if the cauda-specific changes in sperm storage, sperm motility, and epididymal histology following the loss of sympathetic innervation were accompanied by changes in the protein composition of epididymal fluid. One and 4 weeks after surgical IMG removal or sham operations, luminal fluid obtained from the caput and cauda epididymidis and cauda epididymal sperm-associated proteins were subjected to two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and silver-stained proteins were quantitated. One week after IMG removal, two cauda epididymal fluid (CEF) proteins (2 and 13) had increased 43% and 49%, respectively, whereas four CEF proteins (5, 8, 9, and 19) had decreased between 30% and 73% compared to controls. Four weeks after IMG removal, changes in CEF proteins observed 1 week following surgery were no longer present, but the staining intensities of three additional CEF proteins (11, 12, and 18) were reduced an average of 70% compared to control CEF proteins. By obstructing the cauda epididymidis, we confirmed that the changes in CEF protein composition observed following IMG removal were not the result of sperm accumulation but were due directly to the loss of innervation; the staining intensity of CEF protein 2 increased as a result of excessive sperm accumulation in the cauda epididymidis both in the presence and absence of innervation from the IMG. No significant changes in caput epididymal fluid proteins or cauda epididymal sperm-associated proteins were detected following IMG removal. These data show that the protein composition of rat CEF is significantly affected by the loss of sympathetic innervation and suggest that neuronal input may play an important role in the maintenance of epididymal function.
通过手术切除肠系膜下神经节（IMG）对大鼠附睾尾部进行交感神经去支配，会导致附睾尾部精子过度蓄积，以及附睾尾部精子活力和附睾尾部大体组织学的显著变化。本研究的目的是确定交感神经支配丧失后，附睾尾部在精子储存、精子活力和附睾组织学方面的特异性变化是否伴随着附睾液蛋白质组成的变化。在手术切除IMG或假手术后1周和4周，从附睾头和附睾尾获得的管腔液以及附睾尾精子相关蛋白进行二维聚丙烯酰胺凝胶电泳（2-D PAGE），并对银染蛋白进行定量。切除IMG 1周后，两种附睾尾液（CEF）蛋白（2号和13号）分别增加了43%和49%，而与对照组相比，四种CEF蛋白（5号、8号、9号和19号）减少了30%至73%。切除IMG 4周后，术后1周观察到的CEF蛋白变化不再存在，但另外三种CEF蛋白（11号、12号和18号）的染色强度与对照CEF蛋白相比平均降低了70%。通过阻塞附睾尾部，我们证实了切除IMG后观察到的CEF蛋白组成变化不是精子蓄积的结果，而是直接由于神经支配丧失；无论是否存在来自IMG的神经支配，附睾尾部精子过度蓄积都会导致CEF蛋白2的染色强度增加。切除IMG后，附睾头管腔液蛋白或附睾尾精子相关蛋白未检测到显著变化。这些数据表明，大鼠CEF的蛋白质组成受到交感神经支配丧失的显著影响，并表明神经元输入可能在维持附睾功能中起重要作用。
