Pogosian G A, Dremina E S, Sharov V S, Zakarian A E, Panosian G A, Vladimirov Iu A
Biofizika. 1996 Mar-Apr;41(2):342-7.
Kinetics of TBARS accumulation, Fe2+ oxidation, and chemiluminescence were measured in nuclear and mitochondrial membrane suspensions during Fe(2+)-induced lipid peroxidation development. Above certain critical Fe2+ concentrations ([Fe2+].) the latent period (t) of lipid peroxidation development were observed. By using the dependence of t on [Fe2+] the [Fe2+]. values were measured in nuclear and mitochondrial membrane suspensions. At the same concentrations of nuclear and mitochondrial membranes (1 mg/ml) the [Fe2+] value changed from 10 to 30 mu kM. In the case of nuclear membrane suspension the [Fe2+] value changed from 20 to 30 mu kM with the change of membrane concentrations from 0.5 to 1.5 mg/ml. For mitochondrial membrane suspension [Fe2+]. value increased from 10 to 40 mu kM with increasing membrane concentrations from 1 to 4 mg/ml. Apparently, [Fe2+]. value is the main parameter determining the kinetics of Fe(2+)-induced lipid peroxidation in native membrane preparations; the [Fe2+]. value in different membrane systems, in turn, is dependent on the ability of the membrane to bind Fe2+ ions.
在铁（2+）诱导的脂质过氧化发展过程中，对核膜和线粒体膜悬浮液中硫代巴比妥酸反应物（TBARS）积累、Fe2+氧化和化学发光的动力学进行了测量。在高于特定临界Fe2+浓度（[Fe2+]。）时，观察到脂质过氧化发展的潜伏期（t）。通过使用t对[Fe2+]的依赖性，在核膜和线粒体膜悬浮液中测量了[Fe2+]。值。在核膜和线粒体膜浓度相同（1mg/ml）时，[Fe2+]值从10变为30μkM。在核膜悬浮液的情况下，随着膜浓度从0.5mg/ml变为1.5mg/ml，[Fe2+]值从20变为30μkM。对于线粒体膜悬浮液，随着膜浓度从1mg/ml增加到4mg/ml，[Fe2+]。值从10增加到40μkM。显然，[Fe2+]。值是决定天然膜制剂中铁（2+）诱导的脂质过氧化动力学的主要参数；不同膜系统中的[Fe2+]。值又取决于膜结合Fe2+离子的能力。
