Chernitskiĭ E A, Iamaĭkina I V
Biofizika. 1996 Mar-Apr;41(2):417-20.
It is shown that the value of activation energy of red blood cells autohemolysis in temperature range of 4 + 37 degrees C is considerably smaller than that at t > 37 degrees C and is about 50 kJ/mol in isotonic saline buffer, pH 7.4. This means that the change of limiting stage of hemolysis occurs at 37 degrees C. It is found that the decreasing of oxygen partial pressure in the medium resulted in marked inhibition of hemolysis without alteration of activation energy in temperature range of 20 divided by 37 degrees C and on the contrary in intensification of hemolysis at t < 20 degrees C. It is concluded that oxidative processes are essential in autohemolysis in temperature range of 20 divided by 37 degrees C and the oxygen stabilizes the structure state of erythrocytes below 20 degrees C.
结果表明,在4至37摄氏度温度范围内,红细胞自溶的活化能值明显小于t>37摄氏度时的值,在pH值为7.4的等渗盐水缓冲液中约为50 kJ/mol。这意味着溶血极限阶段的变化发生在37摄氏度。研究发现,介质中氧分压的降低导致溶血明显受到抑制,在20至37摄氏度温度范围内活化能没有改变,相反,在t<20摄氏度时溶血加剧。得出的结论是,在20至37摄氏度温度范围内,氧化过程在自溶中至关重要,而在20摄氏度以下,氧可稳定红细胞的结构状态。