Hansen T W, Allen J W
Department of Anesthesia and Critical Care Medicine, University of Pittsburgh, Children's Hospital of Pittsburgh, PA 15213-2583, USA.
Biol Neonate. 1996;69(4):268-74. doi: 10.1159/000244320.
The risk of bilirubin encephalopathy is believed to be increased in hemolytic relative to nonhemolytic jaundice. Young SPRD rats were injected with either acetylphenylhydrazine (APHZ, n = 22) 75 mg/kg or an equivalent volume of the solvent (control, n = 22) intraperitoneally for 3 successive days. One the 4th day, hyperbilirubinemia was induced by a 5-min intravenous infusion of 50 mg/kg bilirubin. After sacrifice (at 10 or 60 min) the brain vessels were flushed in situ with ice-cold saline. Serum bilirubin was 600 +/- 108 mumol/l (mean +/- SD) at 10 min, and 295 +/- 98 mumol/l at 60 min. Hematocrit was significantly reduced in the APHZ rats versus controls (26 +/- 3 vs. 41 +/- 3, p < 0.0001). The concentration of bilirubin in brain was determined by acid chloroform extraction and diazotization. The brain bilirubin values were 5.5 +/- 1.5 versus 5.4 +/- 2.1 mmol/g at 10 min (APHZ vs. control), and 1.3 +/- 0.6 versus 0.8 +/- 0.7 nmol/g at 60 min. The half-life of bilirubin in brain was calculated with an exponential fitting program. The half-life of bilirubin in brain for the two groups was 24.3 +/- 21.9 versus 18.5 +/- 28.3 min. There were no significant differences between the groups in either of these measures. We conclude that in young rats, hemolytic anemia does not increase the acute entry of bilirubin into brain, nor does it affect the clearance of bilirubin from brain.
据信，与非溶血性黄疸相比，溶血性黄疸时胆红素脑病的风险会增加。将年轻的SPRD大鼠连续3天腹腔注射乙酰苯肼（APHZ，n = 22，剂量为75 mg/kg）或等量溶剂（对照组，n = 22）。在第4天，通过静脉输注50 mg/kg胆红素5分钟诱导高胆红素血症。处死后（10分钟或60分钟时），用冰冷盐水原位冲洗脑血管。10分钟时血清胆红素为600±108 μmol/l（平均值±标准差），60分钟时为295±98 μmol/l。与对照组相比，APHZ大鼠的血细胞比容显著降低（26±3对41±3，p < 0.0001）。通过酸氯仿萃取和重氮化法测定脑中胆红素浓度。10分钟时脑胆红素值在APHZ组与对照组分别为5.5±1.5对5.4±2.1 mmol/g，60分钟时分别为1.3±0.6对0.8±0.7 nmol/g。用指数拟合程序计算胆红素在脑中的半衰期。两组胆红素在脑中的半衰期分别为24.3±21.9对18.5±28.3分钟。这些指标在两组之间均无显著差异。我们得出结论，在幼鼠中，溶血性贫血既不会增加胆红素急性进入脑内的量，也不会影响胆红素从脑内的清除。
