Ricci G, Rasperini G, Silvestri M, Cocconcelli P S
Istituto di Discipine Odontostomatologiche, H. San Raffaele, Università degli Studi di Milano.
J Periodontol. 1996 May;67(5):490-6. doi: 10.1902/jop.1996.67.5.490.
The use of membranes for periodontal regeneration is well established. In clinical use, the exposure of membranes to the oral microflora may result in a pathway for periodontal infections. An important role in this process is played by Porphyromonas gingivalis. The purpose of the present study was to examine the colonization of 6 different bioresorbable and nonresorbable membranes for periodontal regeneration by the strain DSM 20709 of P. gingivalis and to determine the time needed by this microorganism to pass through the membranes. A device consisting of a tube sealed with the membranes and filled with a medium suitable for the growth of P. gingivalis was incubated in a bigger tube containing the same medium to study the process of colonization and the crossing of membranes. The outer tube was inoculated with 10(4) cells of P. gingivalis DSM 20709. The passage of bacteria through the membranes was monitored at 6, 24, and 48 hours by counting the number of cells in the inner tube. The colonized membranes were observed using a scanning electron microscope. Differences in the behavior of the 6 membranes analyzed were demonstrated.
牙周再生中膜的使用已得到充分确立。在临床应用中，膜暴露于口腔微生物群可能会导致牙周感染途径。牙龈卟啉单胞菌在这一过程中起着重要作用。本研究的目的是检测牙龈卟啉单胞菌DSM 20709菌株在6种不同的用于牙周再生的生物可吸收和不可吸收膜上的定植情况，并确定该微生物穿过这些膜所需的时间。一个由用膜密封并装有适合牙龈卟啉单胞菌生长的培养基的管子组成的装置，被置于一个装有相同培养基的更大的管子中进行培养，以研究定植过程和膜的穿透情况。外管接种了10⁴个牙龈卟啉单胞菌DSM 20709细胞。通过计算内管中的细胞数量，在6小时、24小时和48小时监测细菌穿过膜的情况。使用扫描电子显微镜观察定植的膜。分析的6种膜的行为差异得到了证实。
