Ross J S, Cheung C, Sheehan C, del Rosario A D, Bui H X, Fisher H A
Department of Pathology, Albany Medical Center, NY 12208, USA.
Diagn Cytopathol. 1996 Jun;14(4):310-5. doi: 10.1002/(SICI)1097-0339(199605)14:4<310::AID-DC6>3.0.CO;2-J.
E-cadherin (E-CD) is a cell-adhesion molecule that has been associated with invasion and metastasis in a wide variety of human neoplasms. We have recently shown that, although decreased E-CD expression is associated with increased bladder-wall invasion and higher tumor grade of infiltrating transitional cell carcinomas (TCC), E-CD expression in the exophytic portion of pure papillary and papillary-infiltrating TCC is increased over that of normal transitional cells. To evaluate whether E-CD levels could serve as a diagnostic adjunct in urinary cytology specimens, we stained 40 alcohol-fixed bladder-washing cytospin preparations with an avidin-biotin-peroxidase method using a monoclonal antibody to E-CD (Sigma Chemical Co., St. Louis, MO). E-CD expression level was defined as a high-intensity or low-intensity staining increase over background squamous cell staining for the transitional cells in 21 biopsy-proven transitional cell carcinomas with papillary components, and in 19 benign or reactive control specimens. Twenty-one of 21 TCC (100%) showed an increased E-CD level over background, with 13 low-intensity and 8 highintensity cases. Ten of 19 benign cases (53%) showed increased E-CD staining over background, with 8 low-intensity and 2 highintensity cases. This difference between malignant and benign specimens was statistically significant (chi-square test. P approximately 0.001). We conclude that increased E-CD expression in the papillary components of TCC can be identified in urinary cytology specimens, may reflect the physical and chemical structural makeup of papillary architecture, and warrants further study as a diagnostic adjunct in the interpretation of urine cytology specimens.
E-钙黏蛋白（E-CD）是一种细胞黏附分子，在多种人类肿瘤中与侵袭和转移相关。我们最近发现，虽然E-CD表达降低与浸润性移行细胞癌（TCC）的膀胱壁浸润增加和肿瘤分级升高有关，但纯乳头状和乳头状浸润性TCC外生性部分的E-CD表达高于正常移行细胞。为了评估E-CD水平是否可作为尿液细胞学标本的诊断辅助指标，我们使用抗E-CD单克隆抗体（Sigma化学公司，密苏里州圣路易斯），通过抗生物素蛋白-生物素-过氧化物酶方法对40份酒精固定的膀胱冲洗细胞离心涂片进行染色。E-CD表达水平定义为在21例经活检证实的有乳头状成分的移行细胞癌以及19例良性或反应性对照标本中，移行细胞相对于背景鳞状细胞染色的高强度或低强度染色增加。21例TCC中有21例（100%）显示E-CD水平高于背景，其中13例为低强度，8例为高强度。19例良性病例中有10例（53%）显示E-CD染色高于背景，其中8例为低强度，2例为高强度。恶性和良性标本之间的这种差异具有统计学意义（卡方检验，P约为0.001）。我们得出结论，TCC乳头状成分中E-CD表达增加可在尿液细胞学标本中识别，可能反映乳头状结构的物理和化学结构组成，并且作为尿液细胞学标本解读的诊断辅助指标值得进一步研究。
