Augustine G J, Burns M E, DeBello W M, Pettit D L, Schweizer F E
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Annu Rev Pharmacol Toxicol. 1996;36:659-701. doi: 10.1146/annurev.pa.36.040196.003303.
Exocytosis is the primary means of cellular secretion. Because exocytosis involves fusion between the plasma membrane and the membrane of secretory vesicles, it is likely that proteins on these two membranes, as well as additional proteins in cellular cytoplasm, mediate exocytosis. Although we know much about the proteins of secretory cells, we still have much to learn about how these proteins participate in exocytosis; in no case has an unambiguous exocytotic function been assigned to any of these proteins. To identify the roles of proteins in exocytosis it is necessary to perturb protein function in living secretory cells. We review a number of perturbation strategies and summarize what this approach has taught us about the functional roles of proteins in exocytosis, concluding with a molecular model of protein dynamics during exocytosis.
胞吐作用是细胞分泌的主要方式。由于胞吐作用涉及质膜与分泌囊泡膜之间的融合，这两种膜上的蛋白质以及细胞质中的其他蛋白质可能介导胞吐作用。尽管我们对分泌细胞的蛋白质了解很多，但对于这些蛋白质如何参与胞吐作用仍有很多需要学习的地方；在任何情况下，都没有明确地将胞吐功能赋予这些蛋白质中的任何一种。为了确定蛋白质在胞吐作用中的作用，有必要在活的分泌细胞中干扰蛋白质的功能。我们回顾了一些干扰策略，并总结了这种方法在胞吐作用中关于蛋白质功能作用所教给我们的内容，最后得出胞吐作用期间蛋白质动力学的分子模型。
