Kashihara K, Ishihara T, Akiyama K, Kuroda S, Morimasa T, Shomori T
Department of Neurology, Okayama University Medical School, Japan.
Psychiatry Clin Neurosci. 1995 Dec;49(5-6):291-4. doi: 10.1111/j.1440-1819.1995.tb01904.x.
In order to elucidate the effect of levodopa and bromocriptine on the DNA-binding activities of transcription factors, AP-1 and CREB DNA-binding activities were investigated using gel-shift assay. Intraperitoneal administration of 100 mg/kg levodopa with 50 mg/kg benserazide in rats increased both AP-1 and CREB DNA-binding activities in the dorsolateral aspect of the striatum. The major proteins composing the increased AP-1 were JunB and JunD. Bromocriptine at doses of 2.5 and 5.0 mg/kg, however, did not increase these binding activities. Present results suggest that levodopa but not bromocriptine induces these transcription-regulating proteins in the striatum with normal dopaminergic functioning.
为阐明左旋多巴和溴隐亭对转录因子DNA结合活性的影响，采用凝胶迁移试验研究了AP-1和CREB的DNA结合活性。给大鼠腹腔注射100mg/kg左旋多巴与50mg/kg苄丝肼，可增加纹状体背外侧的AP-1和CREB DNA结合活性。组成增加的AP-1的主要蛋白质是JunB和JunD。然而，2.5mg/kg和5.0mg/kg剂量的溴隐亭并未增加这些结合活性。目前的结果表明，在多巴胺能功能正常的纹状体中，左旋多巴而非溴隐亭可诱导这些转录调节蛋白。
