Nakamura S, Kiatipattanasakul W, Nakayama H, Ono F, Sakakibara I, Yoshikawa Y, Goto N, Doi K
Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
Exp Anim. 1996 Apr;45(2):199-203. doi: 10.1538/expanim.45.199.
To investigate the role of apolipoprotein E (apo E) in amyloidoses of cynomolgus monkeys, the localization of apo E in cerebral amyloid, including senile plaques and cerebrovascular amyloid, and in islet amyloid was examined immunohistochemically. Mature types of senile plaques with amyloid deposits and cerebrovascular amyloid showed intense immunoreactivity to both antisera to apo E and amyloid beta protein (A beta). In contrast, diffuse plaques without obvious Congophilic amyloid showed weak immunoreactivity to antiserum to apo E, but intense reactivity to antiserum to A beta. In addition, the number of these apo E-positive diffuse plaques was small compared with that of A beta-positive plaques. On the other hand, diabetic islet amyloid that was negative with A beta, reacted intensely with antiserum to apo E. These findings suggest that apo E plays an important role in amyloid fibril formation in several types of amyloidoses.
为研究载脂蛋白E（apo E）在食蟹猴淀粉样变性中的作用，采用免疫组织化学方法检测了apo E在脑淀粉样物质（包括老年斑和脑血管淀粉样物质）以及胰岛淀粉样物质中的定位。有淀粉样沉积物的成熟型老年斑和脑血管淀粉样物质对apo E抗血清和β淀粉样蛋白（Aβ）抗血清均显示出强烈的免疫反应性。相比之下，无明显嗜刚果红淀粉样物质的弥漫性斑块对apo E抗血清显示出弱免疫反应性，但对Aβ抗血清显示出强烈反应性。此外，这些apo E阳性弥漫性斑块的数量与Aβ阳性斑块相比少。另一方面，对Aβ呈阴性的糖尿病胰岛淀粉样物质与apo E抗血清发生强烈反应。这些发现表明apo E在几种类型的淀粉样变性中淀粉样纤维形成中起重要作用。
