Cornelis R
Laboratory for Analytical Chemistry, University of Gent, Belgium.
Ann Clin Lab Sci. 1996 May-Jun;26(3):252-63.
The different chemical species of the trace elements in a living system are determinants for their physiological behaviour. Their study is necessary to improve the understanding of trace element kinetics and metabolism. In a complex matrix, such as biological fluids and tissues, some trace elements will occur as free or mononuclear ions; other as low molecular weight complexes, as reversible or irreversible macromolecular complexes. Speciation investigations entail the separation of the compounds, followed by the measurement of the trace element in the different fractions. Frame-work-procedures are outlined and attention is drawn on the many difficulties that can be encountered. These include the complexity of the matrix, insufficient specificity of the separation of biocompounds, fortuitous contaminations with trace elements, and cutting the original metal-protein binding. State of the art description is given for the speciation studies of AI, As, Cd, Cr, Co, Cu, Hg, Ni, Pb, Pt, Se, Sn, and Zn.
生命系统中微量元素的不同化学形态决定了它们的生理行为。对其进行研究对于增进对微量元素动力学和代谢的理解是必要的。在诸如生物体液和组织这样的复杂基质中，一些微量元素会以游离或单核离子的形式存在；其他的则以低分子量复合物、可逆或不可逆的大分子复合物形式存在。形态分析研究需要先分离化合物，然后测量不同组分中的微量元素。文中概述了基本流程，并提请注意可能遇到的诸多困难。这些困难包括基质的复杂性、生物化合物分离特异性不足、微量元素的偶然污染以及切断原始的金属 - 蛋白质结合。文中给出了铝、砷、镉、铬、钴、铜、汞、镍、铅、铂、硒、锡和锌形态分析研究的最新描述。
