Omi T, Kawanami O, Matsuda K, Tsujii A, Kawai M, Henmi H, Ferrans V J
Pathology and Clinical Research Laboratory, Nippon Medical School, Second Hospital, Kawasaki-shi, Japan.
Burns. 1996 May;22(3):206-11. doi: 10.1016/0305-4179(95)00110-7.
Combined transplantation of skin autograft and allograft was used for the treatment of severe burns. The allografts were obtained from cadavers and were pretreated with 15 per cent glycerol for 2 h at 4 degrees C then frozen at -80 degrees C until used. Patches of autografts were placed over the burns and were covered by a stretched mesh of allografts. Biopsy samples of transplanted skin were obtained from 5 days to 4 weeks after grafting. Sections were examined by histological and immunohistochemical strainings. At 4 days, the epidermal-dermal junction of allografted skin was separated due to migration of epithelial cells derived from autograft epidermis or from skin appendages of recipient dermis. At 2 weeks, dermal fibroblasts and capillaries proliferated in autografts. At 3 weeks, the dermal components of the allograft were covered by epithelial cells from recipient tissue and were invaded by fibroblasts and capillaries. At 4 weeks, allografted skin was replaced by granulation tissue, which mediated the adhesion of the grafts to the underlying tissue. Skin allografts with a freeze-thawing pretreatment provide an appropriate matrix for the epithelial relining and for the growth of granulation tissue in burned skin.
自体皮与异体皮联合移植用于治疗重度烧伤。异体皮取自尸体，在4℃下用15%甘油预处理2小时，然后在-80℃冷冻保存备用。将自体皮片置于烧伤部位，并用拉伸的异体皮网覆盖。在移植后5天至4周获取移植皮肤的活检样本。切片进行组织学和免疫组织化学染色检查。移植后4天，异体皮的表皮-真皮连接处因自体皮表皮或受体真皮皮肤附属器来源的上皮细胞迁移而分离。移植后2周，自体皮中真皮成纤维细胞和毛细血管增生。移植后3周，异体皮的真皮成分被受体组织的上皮细胞覆盖，并被成纤维细胞和毛细血管侵入。移植后4周，异体皮被肉芽组织替代，肉芽组织介导移植物与下方组织的粘连。经过冻融预处理的异体皮为烧伤皮肤的上皮重新衬里和肉芽组织生长提供了合适的基质。
