Ou B, Ohno S, Terada N, Fujii Y, Chen H B, Yamabayashi S, Tsukahara S
Department of Ophthalmology, Yamanashi Medical University, Tamaho, Japan.
Ophthalmic Res. 1996;28(1):29-35. doi: 10.1159/000267870.
The ultrastructure of the cytoskeleton of optic nerve axons in guinea pigs was examined by the quick-freezing and deep-etching (QF-DE) method. The optic nerve tissues were treated with 0.5% saponin before QF. In the replicas, the axoplasm, as observed with conventional ultrathin sections, was seen to be composed of longitudinally oriented microtubules (MT) and neurofilaments (NF). Thin and elaborate cross-linking structures were observed in the interstices between MTs, NFs, and membranous organelles. They consisted of two different types: NF-associated cross-linking structures (about 20-50 nm in length) and MT-associated structures (about 10-20 nm in length), and may play a role in the slow transport of NF and MT and fast transport of organelles, respectively.
采用快速冷冻和深度蚀刻（QF-DE）方法对豚鼠视神经轴突细胞骨架的超微结构进行了研究。在快速冷冻之前，视神经组织用0.5%的皂角苷处理。在复制品中，如同在传统超薄切片中观察到的那样，轴浆被认为是由纵向排列的微管（MT）和神经丝（NF）组成。在微管、神经丝和膜性细胞器之间的间隙中观察到了细小而精细的交联结构。它们由两种不同类型组成：与神经丝相关的交联结构（长度约20-50纳米）和与微管相关的结构（长度约10-20纳米），可能分别在神经丝和微管的慢速运输以及细胞器的快速运输中发挥作用。
