Herrero M B, Viggiano J M, Pérez Martínez S, de Gimeno M F
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Buenos Aires, Argentina.
Reprod Fertil Dev. 1996;8(2):301-4. doi: 10.1071/rd9960301.
The effect of three nitric oxide (NO) synthase inhibitors, L-NG-nitro-arginine (NO2Arg), NG-Nitro-L-arginine methyl ester (L-NAME) and aminoguanidine, on in vitro fertilization in the mouse was examined. Mouse epididymal spermatozoa were capacitated in a medium with or without NO synthase inhibitors. Oocytes were inseminated and the percentage of oocytes with two pronuclei was scored after an 8-h incubation. NO2Arg and L-NAME, but not aminoguanidine, inhibited fertilization. L-NAME inhibited fertilization in a dose-dependent manner, and its effects were stereospecific. The inhibitory effect was neutralized by L-arginine but not by D-arginine. Moreover, D-NAME did not inhibit fertilization. The results suggest that NO synthase activity (presumably of the constitutive type is necessary for spermatozoa to display their full fertilizing ability.
研究了三种一氧化氮（NO）合酶抑制剂，即L-NG-硝基精氨酸（NO2Arg）、NG-硝基-L-精氨酸甲酯（L-NAME）和氨基胍，对小鼠体外受精的影响。将小鼠附睾精子在含有或不含有NO合酶抑制剂的培养基中进行获能处理。使卵母细胞受精，并在孵育8小时后对具有两个原核的卵母细胞百分比进行评分。NO2Arg和L-NAME可抑制受精，但氨基胍无此作用。L-NAME以剂量依赖方式抑制受精，且其作用具有立体特异性。L-精氨酸可中和其抑制作用，而D-精氨酸则不能。此外，D-NAME不抑制受精。结果表明，NO合酶活性（可能是组成型的）对于精子发挥其完全受精能力是必需的。
