Kazazoglou T, Fleischer-Lambropoulos E, Geladopoulos T, Kentroti S, Stefanis C, Vernadakis A
University Mental Health Research Institute, Eginition Hospital, Athens, Greece.
Neurochem Res. 1996 May;21(5):609-14. doi: 10.1007/BF02527760.
In this study, we were interested to compare the responsiveness to growth factors, NGF, b-FGF and EGF and cytokines, IL1 beta, and TNF-alpha, in late passages (74-79) C6 glial cells committed astrocytes and astrocytes of advanced passages (26-28) in cultures derived from aged mouse cerebral hemispheres (MACH). Cultures were grown in either DMEM or chemically defined medium (CDM/TIPS) in order to test the effects of growth factors or cytokines. The activity of glutamine synthetase (GS), a marker for astrocytes, was used as a test parameter. We found that treatment with growth factors increased GS activity in both glial cell culture systems with the exception of EGF in C-6 glial cells. Treatment with cytokines markedly decreased GS activity in the late passage C6 glial cells whereas only TNF-alpha had a similar effect on MACH astrocytes. In view of the generally opposite effects of growth factors and cytokines on GS activity, we speculate that these molecules which are also endogenously present in glial cells may play a role in the maintenance of cellular homeostasis.
在本研究中，我们感兴趣的是比较来自老年小鼠大脑半球（MACH）培养物中晚期传代（74 - 79代）的C6神经胶质细胞来源的星形胶质细胞和晚期传代（26 - 28代）的星形胶质细胞对生长因子（神经生长因子、碱性成纤维细胞生长因子和表皮生长因子）以及细胞因子（白细胞介素1β和肿瘤坏死因子-α）的反应性。培养物在DMEM或化学成分确定的培养基（CDM/TIPS）中生长，以测试生长因子或细胞因子的作用。谷氨酰胺合成酶（GS）的活性作为星形胶质细胞的标志物，用作测试参数。我们发现，生长因子处理可增加两种神经胶质细胞培养系统中的GS活性，但C6神经胶质细胞中的表皮生长因子除外。细胞因子处理显著降低了晚期传代C6神经胶质细胞中的GS活性，而只有肿瘤坏死因子-α对MACH星形胶质细胞有类似作用。鉴于生长因子和细胞因子对GS活性的总体相反作用，我们推测这些在神经胶质细胞中也内源性存在的分子可能在维持细胞内稳态中发挥作用。
