Schmitt E, Mechulam Y, Ruff M, Mitschler A, Moras D, Blanquet S
Laboratorie de Biochimie, Unité de Recherche Associée no. 1970 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.
Proteins. 1996 May;25(1):139-41. doi: 10.1002/prot.14.
Methionyl-tRNA(fMet) formyltransferase from Escherichia coli, a monomer of 34kDa, was overexpressed from its cloned gene fmt (Guillon, J.M., Mechulam, Y., Schmitter, J.M., Blanquet, S., and Fayat, G., J. Bacteriol. 174:4294-4301, 1992) and crystallized using ammonium sulphate as precipitant. The crystals are trigonal and have unit cell parameters a = b = 151.0 A, c = 81.8 A. They belong to space group P3(2)21 and diffract to 2.0 A resolution. The structure is being solved by multiple isomorphous replacement.
来自大肠杆菌的甲硫氨酰 - tRNA（fMet）甲酰基转移酶是一种34kDa的单体，通过其克隆基因fmt进行过表达（Guillon, J.M., Mechulam, Y., Schmitter, J.M., Blanquet, S., and Fayat, G., J. Bacteriol. 174:4294 - 4301, 1992），并使用硫酸铵作为沉淀剂进行结晶。晶体为三角晶系，晶胞参数a = b = 151.0 Å，c = 81.8 Å。它们属于空间群P3(2)21，衍射分辨率为2.0 Å。该结构正在通过多同晶置换法解析。
