与Arc阻遏物相关的寡肽与DNA的相互作用。

Interaction with DNA of oligopeptides related to the Arc repressor.

作者信息

Helbecque N, el Idrissi Boutaher A, Hénichart J P

机构信息

Service d'Epidémiologie et de Santé Publique et INSERM, Institut Pasteur de Lille, France.

出版信息

Pept Res. 1996 Jan-Feb;9(1):21-7.

PMID:8727480

Abstract

Three different peptides, A13, A14x2 and A20, related to the Arc repressor from Salmonella phage P22, were synthesized. They all contained the 13 N-terminal residues of Arc known to form a beta-sheet interacting with the operator OArc. In the case of A20, the tripeptide Lys-Trp-Lys was added in the C-terminal position because of its propensity to increase affinity to DNA. The interaction of the three peptides with OArc and with other related (OMnt from the same phage) and unrelated oligonucleotides was followed using circular dichroism, filter binding assays and DNaseI protection experiments. While Kd = 10(-9) M for the protein, 8.7 x 10(-5) M and 7.7 x 10(-6) M Kd values were obtained for A14x2 and A20 interacting with OArc.

摘要

合成了三种与沙门氏菌噬菌体P22的Arc阻遏物相关的不同肽段，即A13、A14x2和A20。它们都包含Arc的13个N端残基，已知这些残基可形成与操纵子OArc相互作用的β-折叠。就A20而言，由于其具有增加对DNA亲和力的倾向，在C端位置添加了三肽Lys-Trp-Lys。使用圆二色性、滤膜结合测定和DNaseI保护实验来跟踪这三种肽与OArc以及与其他相关（来自同一噬菌体的OMnt）和不相关寡核苷酸的相互作用。蛋白质的解离常数Kd = 10^(-9) M，而A14x2和A20与OArc相互作用时的Kd值分别为8.7×10^(-5) M和7.7×10^(-6) M。