光感受器细胞分化过程中视觉色素和转导蛋白表达的差异。

The differences in the expressions of visual pigments and transducin in photoreceptor cell differentiation.

作者信息

Ohguro H, Kitamura K, Konari K, Sohma H, Fukada Y, Akino T

机构信息

Department of Ophthalmology, Sapporo Medical University, Japan.

出版信息

Tohoku J Exp Med. 1996 Mar;178(3):233-40. doi: 10.1620/tjem.178.233.

DOI:10.1620/tjem.178.233

PMID:8727705

Abstract

The distribution and accumulation of visual pigments, i.e., rod pigment, rhodopsin and red sensitive cone pigment, iodopsin, and transducin in the retina of chicken and chicken embryo were investigated immunohistochemically using their specific antibodies. The immunoreactivities of these proteins appeared at the early stage of photoreceptor differentiation (embryonal day 15) and increased in the photoreceptor cells appeared to reach maximum at the end of the embryonal period (embryonal day 20). On the other hand, although the immunoreactivity of beta gamma subunit of transducin (T beta gamma) was detected at embryonal day 15, the expression level of T beta gamma still remained in low level during the embryonal period. These observations suggest that both T beta gamma and visual pigments are expressed during the embryonic period in chicken photoreceptor cells, but their accumulations in the cells are different.

摘要

利用鸡和鸡胚视网膜中视色素（即视杆色素、视紫红质和红色敏感视锥色素、视锥视紫质）及转导素的特异性抗体，通过免疫组织化学方法研究了它们在鸡和鸡胚视网膜中的分布与积累情况。这些蛋白质的免疫反应性在光感受器分化的早期阶段（胚胎第15天）出现，并在光感受器细胞中增加，在胚胎期结束时（胚胎第20天）似乎达到最大值。另一方面，虽然在胚胎第15天检测到了转导素βγ亚基（Tβγ）的免疫反应性，但在胚胎期Tβγ的表达水平仍处于较低水平。这些观察结果表明，Tβγ和视色素在鸡光感受器细胞的胚胎期均有表达，但它们在细胞中的积累情况不同。