Sharma Y, Balasubramanian D
Centre for Cellular and Molecular Biology, Hyderabad, India.
Ophthalmic Res. 1996;28 Suppl 1:44-7. doi: 10.1159/000267942.
beta-Crystallins, the oligomeric proteins of the eye lens, were found to bind calcium ions with low affinity, in the millimolar range. Calcium was found to induce conformational changes in the secondary and tertiary structure of beta-crystallins. While these changes in conformation are seen in low ionic strength media, they are masked when the protein is dissolved in an intermediate ionic strength medium, where oligomers of lower molecular weight are formed. The calcium binding takes place in these conditions. The behaviour of this protein with respect to calcium binding is reviewed.
β-晶状体蛋白是眼晶状体中的寡聚蛋白，被发现以低亲和力结合钙离子，亲和力范围在毫摩尔级别。研究发现，钙会诱导β-晶状体蛋白二级和三级结构发生构象变化。虽然在低离子强度介质中可以看到这些构象变化，但当蛋白质溶解在中等离子强度介质中时，这些变化会被掩盖，此时会形成较低分子量的寡聚物。钙结合就是在这些条件下发生的。本文综述了这种蛋白质在钙结合方面的行为。
