Buchheim M A, Lemieux C, Otis C, Gutell R R, Chapman R L, Turmel M
Faculty of Biological Science, University of Tulsa, Oklahoma 74104-3189, USA.
Mol Phylogenet Evol. 1996 Apr;5(2):391-402. doi: 10.1006/mpev.1996.0034.
Phylogenetic analyses of nuclear-encoded small-subunit rRNA sequences and chloroplast-encoded large-subunit rRNA sequences from flagellate green algae representing the order Chlamydomonadales were found to show considerable congruence. In general, the chloroplast data set exhibited more robust support for comparable lineages than the nuclear data set. The phylogenetic inferences derived from the independent data sets support some, but also challenge many, traditional taxonomic and phylogenetic concepts regarding the green flagellates. Results from phylogenetic analyses of both molecular data sets support six distinct lineages that include taxa from the biflagellate genus, Chlamydomonas, and a basal lineage that comprises taxa from the quadriflagellate genus, Carteria. Both data sets support the conclusion that Chlamydomonas is not monophyletic. Although the chloroplast data are ambiguous regarding the question of Carteria monophyly, the nuclear data fail to support Carteria monophyly. The chlorococcalean genus Chlorococcum was found to have affinities with the Chlamydomonadales, indicating that the traditional concepts of both Chlorococcales and Chlamydomonadales may need revision. The genus Dunaliella is allied within the Chlamydomonadales, supporting the contention that it has lost a typical glycoprotein cell wall.
对代表衣藻目（Chlamydomonadales）的鞭毛绿藻的核编码小亚基rRNA序列和叶绿体编码大亚基rRNA序列进行的系统发育分析显示出相当程度的一致性。总体而言，与核数据集相比，叶绿体数据集对可比谱系的支持更为有力。从独立数据集得出的系统发育推断支持了一些，但也挑战了许多关于绿鞭毛虫的传统分类学和系统发育概念。两个分子数据集的系统发育分析结果支持六个不同的谱系，其中包括来自双鞭毛属衣藻（Chlamydomonas）的分类群，以及一个基部谱系，该谱系包含来自四鞭毛属卡特藻（Carteria）的分类群。两个数据集都支持衣藻不是单系的这一结论。尽管叶绿体数据在卡特藻单系性问题上不明确，但核数据不支持卡特藻单系性。发现绿球藻属（Chlorococcum）与衣藻目有亲缘关系，这表明绿球藻目和衣藻目的传统概念可能都需要修订。杜氏藻属（Dunaliella）隶属于衣藻目，支持了它已经失去典型糖蛋白细胞壁的观点。
