Salminen O, Lahtinen S, Ahtee L
Department of Pharmacy, University of Helsinki, Finland.
Pharmacol Biochem Behav. 1996 May;54(1):241-8. doi: 10.1016/0091-3057(95)02132-9.
We studied the effects of an acute dose of (-)-nicotine (1 mg/kg) on Fos-like immunostaining (IS) in rat brain areas. Nicotine increased Fos IS significantly in the medial terminal nucleus of accessory optic tract (MT), and tended to increase it in the interpeduncular nucleus (i.p.), as well as in the stress-related areas, the paraventricular hypothalamic nucleus (PVN) and the supraoptic nucleus (SON). Previously nicotine was reported to increase Fos IS also in another stress-related area, the central nucleus of amygdala (ACe). This led us to study the interaction of nicotine with diazepam (10 mg/kg). Diazepam alone increased Fos IS in PVN and in SON as well as in ACe. In diazepam- and nicotine-treated rats Fos IS was increased in PVN and SON as well as in MT and i.p.. In MT and i.p. of diazepam and nicotine-treated rats Fos IS was similar to that induced by nicotine alone, and in PVN and SON of these rats Fos IS in ACe. Taken together, diazepam induced Fos IS in all stress-related areas studied (PVN, SON, ACe), but not in central visual structures, where nicotine induces Fos IS (MT, i.p.). No significant interactions on Fos expression were found between acute effects of diazepam and nicotine suggesting that these drugs activate different sets of neurons within the stress-related brain areas.
我们研究了急性剂量的（-）-尼古丁（1毫克/千克）对大鼠脑区Fos样免疫染色（IS）的影响。尼古丁显著增加了副视束内侧终核（MT）中的Fos IS，并倾向于增加脚间核（i.p.）以及与应激相关区域（室旁下丘脑核（PVN）和视上核（SON））中的Fos IS。先前有报道称尼古丁还会增加另一个与应激相关区域——杏仁核中央核（ACe）中的Fos IS。这促使我们研究尼古丁与地西泮（10毫克/千克）的相互作用。单独使用地西泮会增加PVN、SON以及ACe中的Fos IS。在接受地西泮和尼古丁治疗的大鼠中，PVN、SON以及MT和i.p.中的Fos IS均增加。在接受地西泮和尼古丁治疗的大鼠的MT和i.p.中，Fos IS与单独使用尼古丁诱导的相似，而在这些大鼠的PVN、SON以及ACe中的Fos IS。总体而言，地西泮在所有研究的与应激相关区域（PVN、SON、ACe）中诱导了Fos IS，但在尼古丁诱导Fos IS的中央视觉结构（MT、i.p.）中未诱导。在地西泮和尼古丁的急性作用之间未发现对Fos表达有显著的相互作用，这表明这些药物激活了与应激相关脑区内不同的神经元组。
