Defagot C, Zubin P
Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Biol Res. 1994;27(3-4):193-7.
The present work was intended to evaluate the preparation of antigens, as well as the production and characterization of anti cAMP and cGMP antibodies. Such antibodies were obtained from rabbits, and we used 2'O-succinyl cyclic nucleotide derivate, conjugated with human serum albumin, as antigen. The characterization of the antibodies was monitored by their immunoreactivity with the labelled antigen [125I]-cyclic nucleotide. This assay consists in a competition between a labelled and an unlabelled antigen for a fixed number of binding sites present in the specific antibody. The antibodies were specific for the inducing antigens. Cross-reactivity tests showed low degree competition between the immunogen and other antigens. The very high affinity, high quality and specificity of the generated antibodies indicate that they may be used not only in radioimmunoassay and immunocytochemistry methodologies, but also as bioblockers of physiological pathways.
本研究旨在评估抗原的制备以及抗环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）抗体的产生和特性。此类抗体取自兔子，我们使用与人血清白蛋白偶联的2'-琥珀酰环核苷酸衍生物作为抗原。通过抗体与标记抗原[125I]-环核苷酸的免疫反应性来监测抗体的特性。该检测方法是标记抗原和未标记抗原竞争特异性抗体中固定数量的结合位点。这些抗体对诱导抗原具有特异性。交叉反应测试表明免疫原与其他抗原之间的竞争程度较低。所产生抗体的极高亲和力、高质量和特异性表明，它们不仅可用于放射免疫分析和免疫细胞化学方法，还可作为生理途径的生物阻断剂。
