布宜诺斯艾利斯献血者中人类嗜T淋巴细胞病毒I/II感染情况评估

Evaluation of HTLV-I/II infection in blood donors in Buenos Aires.

作者信息

Gutfraind Z, Blejer J L, Saguier M C, Gómez Carretero M L, Pirola D A, Carreras Vescio L A

机构信息

División Laboratorio Análisis Clínicos, Instituto de Cardiología y Cirugía Cardiovascular, Buenos Aires.

出版信息

Medicina (B Aires). 1995;55(4):295-9.

PMID:8728867

Abstract

HTLV-I and HTLV-II are two related retroviruses that are transmitted by sexual contact, breast feeding, blood transfusion and needle sharing. In this study the prevalence of HTLV-I and HTLV-II was evaluated in voluntary blood donors as a measure of the infection in the general population. Samples were tested by a gelatine particle agglutination test and repeatedly reactive samples were confirmed by Western blot tests (WBT), enriched with recombinant rgp21, rgp461 y rgp4611 proteins, which differentiates HTLV-I and HTLV-II antibodies. Of 19,426 samples, 40 were repeatedly reactive by particle agglutination (0.21%). When analyzed by WBT, 6 met the criteria for HTLV-I (0.036%), 2 for HTLV-II (0.01%) and 1 for HTLV-I/II, 13 samples were indeterminate and 18 were negative. The prevalence is low and comparable to that from non endemic countries. Screening for anti HTLV-I/II antibodies is necessary to prevent transmission through blood transfusions.

摘要

人类嗜T淋巴细胞病毒I型（HTLV-I）和人类嗜T淋巴细胞病毒II型（HTLV-II）是两种相关的逆转录病毒，可通过性接触、母乳喂养、输血和共用针头传播。在本研究中，对自愿献血者进行HTLV-I和HTLV-II的流行率评估，以此作为普通人群感染情况的一项指标。样本通过明胶颗粒凝集试验进行检测，反复呈阳性反应的样本通过蛋白质印迹试验（WBT）进行确认，该试验采用重组rgp21、rgp461和rgp4611蛋白，可区分HTLV-I和HTLV-II抗体。在19426份样本中，有40份通过颗粒凝集试验反复呈阳性反应（0.21%）。经WBT分析，6份符合HTLV-I标准（0.036%），2份符合HTLV-II标准（0.01%），1份符合HTLV-I/II标准，13份样本结果不确定，18份为阴性。流行率较低，与非流行国家相当。筛查抗HTLV-I/II抗体对于预防输血传播至关重要。